Steppogenin exhibits significant tyrosinase inhibition activity, ;it also shows strong mushroom tyrosinase inhibitory activity with IC(50) values lower than 50 microM, more potent than kojic acid (IC(50) = 71.6 microM), a well-known tyrosinase inhibitor.

ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.

Alpha-Chaconine has anti-inflammatory effect, associated with the suppression of AP-1, and supports its possible therapeutic role for the treatment of sepsis. Alpha-Chaconine also has cytotoxic effects.